5k7d

The structure of native pistol ribozyme, bound to Iridium

spinqualitylabelsall models 5k7d, resolution 2.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5k7d is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: IRI, MG Related: 5k7c, 5k7e Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The field of small self-cleaving nucleolytic ribozymes has been invigorated by the recent discovery of the twister, twister-sister, pistol and hatchet ribozymes. We report the crystal structure of a pistol ribozyme termed env25, which adopts a compact tertiary architecture stabilized by an embedded pseudoknot fold. The G-U cleavage site adopts a splayed-apart conformation with in-line alignment of the modeled 2'-O of G for attack on the adjacent to-be-cleaved P-O5' bond. Highly conserved residues G40 (N1 position) and A32 (N3 and 2'-OH positions) are aligned to act as a general base and a general acid, respectively, to accelerate cleavage chemistry, with their roles confirmed by cleavage assays on variants, and an increased pKa of 4.7 for A32. Our structure of the pistol ribozyme defined how the overall and local topologies dictate the in-line alignment at the G-U cleavage site, with cleavage assays on variants revealing key residues that participate in acid-base-catalyzed cleavage chemistry. Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage.,Ren A, Vusurovic N, Gebetsberger J, Gao P, Juen M, Kreutz C, Micura R, Patel DJ Nat Chem Biol. 2016 Jul 11. doi: 10.1038/nchembio.2125. PMID:27398999 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribozyme

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