1dyq: Difference between revisions
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[[Image:1dyq.gif|left|200px]]<br /> | [[Image:1dyq.gif|left|200px]]<br /><applet load="1dyq" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1dyq" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1dyq, resolution 1.50Å" /> | caption="1dyq, resolution 1.50Å" /> | ||
'''STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE'''<br /> | '''STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1DYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. | 1DYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=ZN1:Zn Binding Site The Zn Ion Is Bound To Residues HIS A 18 ...'>ZN1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: vaccine]] | [[Category: vaccine]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:47:07 2007'' | ||
Revision as of 12:37, 18 December 2007
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STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE
Overview
Staphylococcal enterotoxins (SEs) are superantigenic protein toxins, responsible for a number of life-threatening diseases. The X-ray structure, of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and, Y92A) vaccine reveals a cascade of structural rearrangements located in, three loop regions essential for binding the alpha subunit of major, histocompatibility complex class II (MHC-II) molecules. A comparison of, hypothetical model complexes between SEA and the SEA triple mutant with, MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic, interactions necessary for forming the complex. Extensive dislocation of, the disulfide loop in particular interferes with MHC-IIalpha binding. The, triple-mutant structure provides new insights into the loss of, superantigenicity and toxicity of an engineered superantigen and provides, a basis for further design of enterotoxin vaccines.
About this Structure
1DYQ is a Single protein structure of sequence from Staphylococcus aureus with ZN and SO4 as ligands. Known structural/functional Site: ZN1. Full crystallographic information is available from OCA.
Reference
Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-IIalpha., Krupka HI, Segelke BW, Ulrich RG, Ringhofer S, Knapp M, Rupp B, Protein Sci. 2002 Mar;11(3):642-51. PMID:11847286
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