5lfp: Difference between revisions

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'''Unreleased structure'''


The entry 5lfp is ON HOLD until Paper Publication
==Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P6322, SeMet)==
<StructureSection load='5lfp' size='340' side='right' caption='[[5lfp]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5lfp]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LFP FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lfj|5lfj]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfp OCA], [http://pdbe.org/5lfp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lfp RCSB], [http://www.ebi.ac.uk/pdbsum/5lfp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfp ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/BPA_MYCTU BPA_MYCTU]] Interacts with the core proteasome alpha-subunit (PrcA) through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif). Interaction of Bpa with the proteasome stimulates proteosomal peptidase and casein degradation activity, which suggests Bpa could play a role in the removal of non-native or damaged proteins by influencing the conformation of the proteasome complex upon interaction. Can inhibit degradation of Pup-tagged substrates in vitro by competing with Mpa for association with the proteasome.<ref>PMID:25469515</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium tuberculosis harbors proteasomes that recruit substrates for degradation through an ubiquitin-like modification pathway. Recently, a non-ATPase activator termed Bpa (bacterial proteasome activator) was shown to support an alternate proteasomal degradation pathway. Here, we present the cryo-electron microscopy (cryo-EM) structure of Bpa in complex with the 20S core particle (CP). For docking into the cryo-EM density, we solved the X-ray structure of Bpa, showing that it forms tight four-helix bundles arranged into a 12-membered ring with a 40 A wide central pore and the C-terminal helix of each protomer protruding from the ring. The Bpa model was fitted into the cryo-EM map of the Bpa-CP complex, revealing its architecture and striking symmetry mismatch. The Bpa-CP interface was resolved to 3.5 A, showing the interactions between the C-terminal GQYL motif of Bpa and the proteasome alpha-rings. This docking mode is related to the one observed for eukaryotic activators with features specific to the bacterial complex.


Authors: Bolten, M., Delley, C.L., Leibundgut, M., Boehringer, D., Ban, N., Weber-Ban, E.
Structural Analysis of the Bacterial Proteasome Activator Bpa in Complex with the 20S Proteasome.,Bolten M, Delley CL, Leibundgut M, Boehringer D, Ban N, Weber-Ban E Structure. 2016 Dec 6;24(12):2138-2151. doi: 10.1016/j.str.2016.10.008. Epub 2016, Nov 10. PMID:27839949<ref>PMID:27839949</ref>


Description: Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P6322, SeMet)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Delley, C.L]]
<div class="pdbe-citations 5lfp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ban, N]]
[[Category: Boehringer, D]]
[[Category: Boehringer, D]]
[[Category: Bolten, M]]
[[Category: Delley, C L]]
[[Category: Leibundgut, M]]
[[Category: Leibundgut, M]]
[[Category: Ban, N]]
[[Category: Bolten, M]]
[[Category: Weber-Ban, E]]
[[Category: Weber-Ban, E]]
[[Category: Chaperone]]
[[Category: Dodecamer]]
[[Category: Four-helix bundle]]
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