5loz: Difference between revisions
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==STRUCTURE OF YEAST ENT1 ENTH DOMAIN== | |||
<StructureSection load='5loz' size='340' side='right' caption='[[5loz]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5loz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LOZ FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hoa|1hoa]], [[1edu|1edu]], [[1eyh|1eyh]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5loz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5loz OCA], [http://pdbe.org/5loz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5loz RCSB], [http://www.ebi.ac.uk/pdbsum/5loz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5loz ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/ENT1_YEAST ENT1_YEAST]] Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.<ref>PMID:10449404</ref> <ref>PMID:11694597</ref> <ref>PMID:12529323</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Members of the Epsin protein family regulate the ubiquitin/clathrin-dependent trafficking of transmembrane proteins. The yeast Epsin-1 (ent1) gene was cloned and expressed in Escherichia coli. The protein product of a construct containing the ENTH-UIM modules was purified to homogeneity and subjected to crystallization screening using the sitting-drop vapour-diffusion method. Refined conditions containing polyethylene glycol 3350 and Tacsimate yielded thin rod-like crystals. X-ray analysis revealed that the crystallographic symmetry is primitive orthorhombic, space group P222, with unit-cell parameters a = 32.7, b = 35.5, c = 110.6 A and a diffraction limit of 2.3 A. Matthews coefficient calculations suggested that the crystal contained only the ENTH domain. This was corroborated by Coomassie Blue-stained SDS-PAGE analysis of dissolved crystals. | |||
Purification and crystallization of yeast Ent1 ENTH domain.,Tanner N, Prag G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):820-3. doi:, 10.1107/S1744309112022488. Epub 2012 Jun 28. PMID:22750874<ref>PMID:22750874</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5loz" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Prag, G]] | [[Category: Prag, G]] | ||
[[Category: Tanner, N]] | [[Category: Tanner, N]] | ||
[[Category: Alpha-alpha superhelix]] | |||
[[Category: Clathrin]] | |||
[[Category: Endocytosis adaptor]] | |||
[[Category: Eps15]] | |||
[[Category: Lipid]] | |||
[[Category: Ubiquitin]] | |||
[[Category: Ubiquitin receptor]] | |||
[[Category: Ubiquitin-binding domain]] | |||
Revision as of 21:39, 5 October 2016
STRUCTURE OF YEAST ENT1 ENTH DOMAIN
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