1ouz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ouz.gif|left|200px]] | [[Image:1ouz.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1ouz", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1ouz| PDB=1ouz | SCENE= }} | |||
}} | |||
'''Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)''' | '''Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)''' | ||
| Line 30: | Line 27: | ||
[[Category: Read, E K.]] | [[Category: Read, E K.]] | ||
[[Category: Rice, P A.]] | [[Category: Rice, P A.]] | ||
[[Category: | [[Category: Dna bending]] | ||
[[Category: | [[Category: Ihf]] | ||
[[Category: | [[Category: Indirect readout]] | ||
[[Category: | [[Category: Protein-dna recognition]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:18:42 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 01:18, 3 May 2008
Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)
Overview
Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.
About this Structure
1OUZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466 Page seeded by OCA on Sat May 3 04:18:42 2008
