1p58: Difference between revisions
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{{STRUCTURE_1p58| PDB=1p58 | SCENE= }} | |||
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'''Complex Organization of Dengue Virus Membrane Proteins as Revealed by 9.5 Angstrom Cryo-EM reconstruction''' | '''Complex Organization of Dengue Virus Membrane Proteins as Revealed by 9.5 Angstrom Cryo-EM reconstruction''' | ||
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[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: | [[Category: Cryo-em]] | ||
[[Category: | [[Category: Dengue virus]] | ||
[[Category: | [[Category: Flaviviridae]] | ||
[[Category: | [[Category: Flavivirus]] | ||
[[Category: | [[Category: Glycoprotein e from tick-borne encephalitis virus]] | ||
[[Category: | [[Category: Icosahedral virus]] | ||
[[Category: | [[Category: Membrane protein m]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:42:08 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 01:42, 3 May 2008
Complex Organization of Dengue Virus Membrane Proteins as Revealed by 9.5 Angstrom Cryo-EM reconstruction
Overview
Improved technology for reconstructing cryo-electron microscopy (cryo-EM) images has now made it possible to determine secondary structural features of membrane proteins in enveloped viruses. The structure of mature dengue virus particles was determined to a resolution of 9.5 A by cryo-EM and image reconstruction techniques, establishing the secondary structural disposition of the 180 envelope (E) and 180 membrane (M) proteins in the lipid envelope. The alpha-helical 'stem' regions of the E molecules, as well as part of the N-terminal section of the M proteins, are buried in the outer leaflet of the viral membrane. The 'anchor' regions of E and the M proteins each form antiparallel E-E and M-M transmembrane alpha-helices, leaving their C termini on the exterior of the viral membrane, consistent with the predicted topology of the unprocessed polyprotein. This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus.
About this Structure
1P58 is a Protein complex structure of sequences from Dengue virus 2. Full crystallographic information is available from OCA.
Reference
Visualization of membrane protein domains by cryo-electron microscopy of dengue virus., Zhang W, Chipman PR, Corver J, Johnson PR, Zhang Y, Mukhopadhyay S, Baker TS, Strauss JH, Rossmann MG, Kuhn RJ, Nat Struct Biol. 2003 Nov;10(11):907-12. Epub 2003 Oct 5. PMID:14528291 Page seeded by OCA on Sat May 3 04:42:08 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Dengue virus 2
- Protein complex
- Baker, T S.
- Chipman, P R.
- Corver, J.
- Johnson, P R.
- Kuhn, R J.
- Mukhopadhyay, S.
- Rossmann, M G.
- Strauss, J H.
- Zhang, W.
- Zhang, Y.
- Cryo-em
- Dengue virus
- Flaviviridae
- Flavivirus
- Glycoprotein e from tick-borne encephalitis virus
- Icosahedral virus
- Membrane protein m
