1p5v: Difference between revisions
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{{STRUCTURE_1p5v| PDB=1p5v | SCENE= }} | |||
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'''X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex''' | '''X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex''' | ||
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[[Category: Pudney, A F.]] | [[Category: Pudney, A F.]] | ||
[[Category: Zavialov, A V.]] | [[Category: Zavialov, A V.]] | ||
[[Category: | [[Category: Chaperone]] | ||
[[Category: | [[Category: Chaperone-subunit complex]] | ||
[[Category: | [[Category: Chaperone-target complex]] | ||
[[Category: | [[Category: Donor strand complementation]] | ||
[[Category: | [[Category: Donor strand exchange]] | ||
[[Category: | [[Category: Protein fiber]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:43:23 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 01:43, 3 May 2008
X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex
Overview
Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.
About this Structure
1P5V is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.
Reference
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:12787500 Page seeded by OCA on Sat May 3 04:43:23 2008
