5eb4: Difference between revisions

Revision as of 12:32, 16 November 2017

The crystal structure of almond HNL, PaHNL5 V317A, expressed in Aspergillus niger

Structural highlights

5eb4 is a 2 chain structure with sequence from Almond. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	FAD, NAG
NonStd Res:	UNK
Gene:	hnl5 (Almond)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Hydroxynitrile lyases (HNLs) catalyze the asymmetric addition of HCN to aldehydes producing enantiomerically pure cyanohydrins. These enzymes can be heterologously expressed in large quantities making them interesting candidates for industrial applications. The HNLs from Rosaceae evolved from flavin dependent dehydrogenase/oxidase structures. Here we report the high resolution X-ray structure of the highly glycosylated Prunus amygdalus HNL isoenzyme5 (PaHNL5 V317A) expressed in Aspergillus niger and its complex with benzyl alcohol. A comparison with the structure of isoenzyme PaHNL1 indicates a higher accessibility to the active site and a larger cavity for PaHNL5. Additionally, the PaHNL5 complex structure with benzyl alcohol was compared with the structurally related aryl-alcohol oxidase (AAO). Even though both enzymes contain an FAD-cofactor and histidine residues at crucial positions in the active site, PaHNL5 lacks the oxidoreductase activity. The structures indicate that in PaHNLs benzyl alcohol is bound too far away from the FAD cofactor in order to be oxidized.

Structures of almond hydroxynitrile lyase isoenzyme 5 provide a rationale for the lack of oxidoreductase activity in flavin dependent HNLs.,Pavkov-Keller T, Bakhuis J, Steinkellner G, Jolink F, Keijmel E, Birner-Gruenberger R, Gruber K J Biotechnol. 2016 Apr 8. pii: S0168-1656(16)30193-6. doi:, 10.1016/j.jbiotec.2016.04.013. PMID:27067080^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pavkov-Keller T, Bakhuis J, Steinkellner G, Jolink F, Keijmel E, Birner-Gruenberger R, Gruber K. Structures of almond hydroxynitrile lyase isoenzyme 5 provide a rationale for the lack of oxidoreductase activity in flavin dependent HNLs. J Biotechnol. 2016 Apr 8. pii: S0168-1656(16)30193-6. doi:, 10.1016/j.jbiotec.2016.04.013. PMID:27067080 doi:https://dx.doi.org/10.1016/j.jbiotec.2016.04.013

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OCA

[1] Pavkov-Keller T, Bakhuis J, Steinkellner G, Jolink F, Keijmel E, Birner-Gruenberger R, Gruber K. Structures of almond hydroxynitrile lyase isoenzyme 5 provide a rationale for the lack of oxidoreductase activity in flavin dependent HNLs. J Biotechnol. 2016 Apr 8. pii: S0168-1656(16)30193-6. doi:, 10.1016/j.jbiotec.2016.04.013. PMID:27067080 doi:https://dx.doi.org/10.1016/j.jbiotec.2016.04.013

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5eb4' size='340' side='right' caption='[[5eb4]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5eb4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EB4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5eb4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Almond Almond]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EB4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hnl5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3755 Almond])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eb4 OCA], [http://pdbe.org/5eb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eb4 RCSB], [http://www.ebi.ac.uk/pdbsum/5eb4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eb4 ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Almond]]
 [[Category: Gruber, K]]
 [[Category: Pavkov-Keller, T]]

5eb4: Difference between revisions

Revision as of 12:32, 16 November 2017

The crystal structure of almond HNL, PaHNL5 V317A, expressed in Aspergillus niger

Structural highlights

Publication Abstract from PubMed

References

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