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[[Image:1pio.jpg|left|200px]]
[[Image:1pio.jpg|left|200px]]


{{Structure
<!--
|PDB= 1pio |SIZE=350|CAPTION= <scene name='initialview01'>1pio</scene>, resolution 2.8&Aring;
The line below this paragraph, containing "STRUCTURE_1pio", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
|LIGAND=
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
or leave the SCENE parameter empty for the default display.
|GENE= BLAZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
-->
|DOMAIN=
{{STRUCTURE_1pio|  PDB=1pio |  SCENE= }}  
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pio OCA], [http://www.ebi.ac.uk/pdbsum/1pio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pio RCSB]</span>
}}


'''AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS'''
'''AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS'''
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[[Category: Herzberg, O.]]
[[Category: Herzberg, O.]]
[[Category: Zawadzke, L E.]]
[[Category: Zawadzke, L E.]]
[[Category: hydrolase (acting on cyclic amides)]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:07:35 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:00:10 2008''

Revision as of 02:07, 3 May 2008

File:1pio.jpg

Template:STRUCTURE 1pio

AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS


Overview

The beta-lactamase from Staphylococcus aureus PC1 has been cloned into an Escherichia coli vector for site-directed mutagenesis and high-level protein expression. A mutant enzyme has been produced in which Ala238 is replaced by a serine, and Ile239 is deleted (A238S:I239del). The engineered enzyme hydrolyses third-generation cephalosporins substantially more rapidly than the parental enzyme does, while hydrolysis of benzylpenicillin is slower with the mutant than with the wild-type and native enzymes. The mutant beta-lactamase has been crystallized and the structure determined and refined at 2.8 A resolution. The disposition of the beta-strand which forms the side of the active site is altered in comparison with the native S. aureus beta-lactamase structure, widening the active site cleft and providing space to accommodate the bulky side-chains of the third-generation cephalosporins.

About this Structure

1PIO is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins., Zawadzke LE, Smith TJ, Herzberg O, Protein Eng. 1995 Dec;8(12):1275-85. PMID:8869640 Page seeded by OCA on Sat May 3 05:07:35 2008

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