5gnd: Difference between revisions

Revision as of 06:47, 13 May 2020

Structure of Deg protease HhoA from Synechocystis sp. PCC 6803

Structural highlights

5gnd is a 2 chain structure with sequence from Escherichia coli bl21(de3) and Syny3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NA, ZN
NonStd Res:	UNK
Related:	5b6l
Gene:	hhoA (SYNY3)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HHOA_SYNY3] A putative protease, its function overlaps that of the related putative proteases HhoB and HtrA.^[1]

Publication Abstract from PubMed

The high temperature requirement A (HtrA) proteases are oligomeric serine proteases essential for protein quality control. HtrA homolog A (HhoA) from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 assembles into a proteolytically active hexamer. Herein, we present the crystal structure of the hexameric HhoA in complex with the copurified peptide. Our data indicate the presence of three methionines in close proximity to the peptide-binding site of the PDZ domain. Unexpectedly, we observed that a zinc ion is accommodated within the central channel formed by a HhoA trimer. However, neither calcium nor magnesium showed affinity for HhoA. The role of the zinc ion in HhoA was tested in an in vitro proteolytic assay against the nonspecific substrate beta-casein and was found to be inhibitory. Our findings provide insights into the regulation of HhoA by a redox-related mechanism involving methionine residues and by zinc ion-binding within the central channel.

Crystal structure of the zinc-bound HhoA protease from Synechocystis sp. PCC 6803.,Dong W, Wang J, Niu G, Zhao S, Liu L FEBS Lett. 2016 Oct;590(19):3435-3442. doi: 10.1002/1873-3468.12416. Epub 2016, Sep 23. PMID:27616292^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Barker M, de Vries R, Nield J, Komenda J, Nixon PJ. The deg proteases protect Synechocystis sp. PCC 6803 during heat and light stresses but are not essential for removal of damaged D1 protein during the photosystem two repair cycle. J Biol Chem. 2006 Oct 13;281(41):30347-55. Epub 2006 Aug 15. PMID:16912048 doi:https://dx.doi.org/10.1074/jbc.M601064200
↑ Dong W, Wang J, Niu G, Zhao S, Liu L. Crystal structure of the zinc-bound HhoA protease from Synechocystis sp. PCC 6803. FEBS Lett. 2016 Oct;590(19):3435-3442. doi: 10.1002/1873-3468.12416. Epub 2016, Sep 23. PMID:27616292 doi:https://dx.doi.org/10.1002/1873-3468.12416

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Barker M, de Vries R, Nield J, Komenda J, Nixon PJ. The deg proteases protect Synechocystis sp. PCC 6803 during heat and light stresses but are not essential for removal of damaged D1 protein during the photosystem two repair cycle. J Biol Chem. 2006 Oct 13;281(41):30347-55. Epub 2006 Aug 15. PMID:16912048 doi:https://dx.doi.org/10.1074/jbc.M601064200

[2] Dong W, Wang J, Niu G, Zhao S, Liu L. Crystal structure of the zinc-bound HhoA protease from Synechocystis sp. PCC 6803. FEBS Lett. 2016 Oct;590(19):3435-3442. doi: 10.1002/1873-3468.12416. Epub 2016, Sep 23. PMID:27616292 doi:https://dx.doi.org/10.1002/1873-3468.12416

[1]

[2]

@@ Line 1: / Line 1: @@
 ==Structure of Deg protease HhoA from Synechocystis sp. PCC 6803==
-<StructureSection load='5gnd' size='340' side='right' caption='[[5gnd]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='5gnd' size='340' side='right'caption='[[5gnd]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5gnd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GND OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GND FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5gnd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)] and [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GND OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GND FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5b6l|5b6l]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gnd OCA], [http://pdbe.org/5gnd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gnd RCSB], [http://www.ebi.ac.uk/pdbsum/5gnd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gnd ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hhoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gnd OCA], [http://pdbe.org/5gnd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gnd RCSB], [http://www.ebi.ac.uk/pdbsum/5gnd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gnd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Syny3]]
 [[Category: Dong, W]]
 [[Category: Liu, L]]

5gnd: Difference between revisions

Revision as of 06:47, 13 May 2020

Structure of Deg protease HhoA from Synechocystis sp. PCC 6803

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools