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'''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1''' | '''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1''' | ||
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[[Category: Berg, J M.]] | [[Category: Berg, J M.]] | ||
[[Category: Berkovits-Cymet, H J.]] | [[Category: Berkovits-Cymet, H J.]] | ||
[[Category: | [[Category: Cchhc zinc binding domain]] | ||
[[Category: | [[Category: Dna binding domain]] | ||
[[Category: | [[Category: Neural zinc finger factor-1]] | ||
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Revision as of 02:35, 3 May 2008
Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1
Overview
The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 (NZF-1) has been determined in solution though the use of NMR methods. This domain is a member of a family of domains that have the Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure determination reveals a novel fold based around a zinc(II) ion coordinated to three Cys residues and the second of the two conserved His residues. The other His residue is stacked between the metal-coordinated His residue and a relatively conserved aromatic residue. Analysis of His to Gln sequence variants reveals that both His residues are required for the formation of a well-defined structure, but neither is required for high-affinity metal binding at a tetrahedral site. The structure suggests that a two-domain protein fragment and a double-stranded DNA binding site may interact with a common two-fold axis relating the two domains and the two half-sites of the DNA-inverted repeat.
About this Structure
1PXE is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding., Berkovits-Cymet HJ, Amann BT, Berg JM, Biochemistry. 2004 Feb 3;43(4):898-903. PMID:14744132 Page seeded by OCA on Sat May 3 05:35:57 2008
