3rlb

Crystal structure at 2.0 A of the S-component for thiamin from an ECF-type ABC transporter

spinqualitylabelsall models 3rlb, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3rlb is a 2 chain structure with sequence from Lacln. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BNG, VIB Gene: LLNZ_01755 (LACLN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Energy coupling factor (ECF) transporters are used for the uptake of vitamins in Prokarya. They consist of an integral membrane protein that confers substrate specificity (the S-component) and an energizing module that is related to ATP-binding cassette (ABC) transporters. S-components for different substrates often do not share detectable sequence similarity but interact with the same energizing module. Here we present the crystal structure of the thiamine-specific S-component ThiT from Lactococcus lactis at 2.0 A. Extensive protein-substrate interactions explain its high binding affinity for thiamine (K(d) ~10(-10) M). ThiT has a fold similar to that of the riboflavin-specific S-component RibU, with which it shares only 14% sequence identity. Two alanines in a conserved motif (AxxxA) located on the membrane-embedded surface of the S-components mediate the interaction with the energizing module. Based on these findings, we propose a general transport mechanism for ECF transporters. The structural basis of modularity in ECF-type ABC transporters.,Erkens GB, Berntsson RP, Fulyani F, Majsnerowska M, Vujicic-Zagar A, Ter Beek J, Poolman B, Slotboom DJ Nat Struct Mol Biol. 2011 Jun 26;18(7):755-60. doi: 10.1038/nsmb.2073. PMID:21706007 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ABC transporter 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA