4h1s

Crystal Structure of a Truncated Soluble form of Human CD73 with Ecto-5'-Nucleotidase activity

spinqualitylabelsall models 4h1s, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4h1s is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAG, PO4, ZN Activity: 5'-nucleotidase, with EC number 3.1.3.5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease [5NTD_HUMAN] Hereditary arterial and articular multiple calcification syndrome. The disease is caused by mutations affecting the gene represented in this entry. Function [5NTD_HUMAN] Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. Publication Abstract from PubMed CD73 is a dimeric ecto-5'-nucleotidase that is expressed on the exterior side of the plasma membrane. CD73 has important regulatory functions in the extracellular metabolism of certain nucleoside monophosphates, in particular adenosine monophosphate, and has been linked to a number of pathological conditions such as cancer and myocardial ischaemia. Here, we present the crystal structure of a soluble form of human soluble CD73 (sCD73) at 2.2 A resolution, a truncated form of CD73 that retains ecto-5'-nucleotidase activity. With this structure we obtained insight into the dimerisation of CD73, active site architecture, and a sense of secondary modifications of the protein. The crystal structure reveals a conserved loop that is directly involved in the dimer-dimer interaction showing that the two subunits of the dimer are not linked by disulfide bridges. Using biophotonic microarray imaging we were able to confirm glycosylation of the enzyme and show that the enzyme is decorated with a variety of oligosaccharide structures. The crystal structure of sCD73 will aid the design of inhibitors or activator molecules for the treatment of several diseases and prove useful in explaining the possible roles of single nucleotide polymorphisms in physiology and disease. Crystal Structure of a Soluble Form of Human CD73 with Ecto-5'-Nucleotidase Activity.,Heuts DP, Weissenborn MJ, Olkhov RV, Shaw AM, Levy C, Scrutton NS Chembiochem. 2012 Sep 20. doi: 10.1002/cbic.201200426. PMID:22997138 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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