4gf4

Low pH structure of Pseudomonas putida OprB

spinqualitylabelsall models 4gf4, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4gf4 is a 1 chain structure with sequence from Psepb. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: C8E NonStd Res: MSE Related: 4gey Gene: PPUBIRD1_1069 (PSEPB) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Substrate-specific outer membrane (OM) channels of Gram-negative bacteria mediate uptake of many small molecules, including carbohydrates. The mechanism of sugar uptake by enterobacterial channels such as E. coli LamB (maltoporin) has been characterized in great detail. In pseudomonads and related organisms, sugar uptake is not mediated by LamB, but by OprB channels. Beyond the notion that OprB channels seem to prefer monosaccharides as substrates, very little is known about OprB-mediated sugar uptake. Here I report the X-ray crystal structure of an OprB channel from Pseudomonas putida F1. The structure shows that OprB forms a monomeric, 16-stranded beta-barrel with a constriction formed by extracellular loops L2 and L3. The side chains of two highly conserved arginine residues (Arg83 and Arg110) and a conserved glutamate (Glu106) line the channel constriction and interact with a bound glucose molecule. Liposome swelling uptake assays show a strong preference for monosaccharide transport over disaccharides. Moreover, substrates with a net negative charge are disfavored by the channel, likely due to the negatively charged character of the constriction. The architecture of the eyelet and the absence of a greasy slide provide an explanation for the observed specificity of OprB for monosaccharides rather than the oligosaccharides preferred by LamB and related enterobacterial channels. Structural basis for outer membrane sugar uptake in pseudomonads.,van den Berg B J Biol Chem. 2012 Oct 12. PMID:23066028 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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