5wty: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "5wty" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
The | ==Structure of Nop9 RNA complex== | ||
<StructureSection load='5wty' size='340' side='right' caption='[[5wty]], [[Resolution|resolution]] 2.79Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5wty]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTY FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wtx|5wtx]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wty OCA], [http://pdbe.org/5wty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wty RCSB], [http://www.ebi.ac.uk/pdbsum/5wty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wty ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST]] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The assembly of eukaryotic ribosomes requires numerous factors that transiently associate with evolving pre-ribosomal particles. The Pumilio repeat-containing protein Nop9 briefly associates with the 90S pre-ribosome during its co-transcriptional assembly. Here, we show that Nop9 specifically binds an 11-nucleotide sequence of 18S rRNA that forms the 3' side of the central pseudoknot and helix 28 in the mature subunit. Crystal structures of Nop9 in the free and RNA-bound states reveal a new type of Pumilio repeat protein with a distinct structure, target sequence and RNA-binding mode. Nop9 contains 10 Pumilio repeats arranged into a U-shaped scaffold. The target RNA is recognized by two stretches of repeats in a bipartite manner, and three central bases are unrecognized as a result of the degeneracy of repeats 6 and 7. Our data suggest that Nop9 regulates the folding of 18S rRNA at early assembly stages of 90S. | |||
Nop9 binds the central pseudoknot region of 18S rRNA.,Wang B, Ye K Nucleic Acids Res. 2017 Jan 3. pii: gkw1323. doi: 10.1093/nar/gkw1323. PMID:28053123<ref>PMID:28053123</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5wty" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Wang, B]] | [[Category: Wang, B]] | ||
[[Category: Ye, K]] | [[Category: Ye, K]] | ||
[[Category: Pumilio repeat-containing protein]] | |||
[[Category: Rna binding protein-rna complex]] | |||
[[Category: Rna-binding protein]] | |||
[[Category: Rnp]] | |||
