4i3x

Structure of phosphonoacetaldehyde dehydrogenase in complex with phosphonoacetate and cofactor NAD+

spinqualitylabelsall models 4i3x, resolution 2.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4i3x is a 8 chain structure with sequence from Ensifer meliloti. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAD, PAE Related: 4i3t, 4i3u, 4i3v, 4i3w Gene: phnY, RB0979, SM_b21539 (Ensifer meliloti) Activity: Aldehyde dehydrogenase (NAD(+)), with EC number 1.2.1.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Phosphonates (C-PO3(2-)) have applications as antibiotics, herbicides, and detergents. In some environments, these molecules represent the predominant source of phosphorus, and several microbes have evolved dedicated enzymatic machineries for phosphonate degradation. For example, most common naturally occurring phosphonates can be catabolized to either phosphonoacetaldehyde or phosphonoacetate, which can then be hydrolyzed to generate inorganic phosphate and acetaldehyde or acetate, respectively. The phosphonoacetaldehyde oxidase gene (phnY) links these two hydrolytic processes and provides a previously unknown catabolic mechanism for phosphonoacetate production in the microbial metabolome. Here, we present biochemical characterization of PhnY and high-resolution crystal structures of the apo state, as well as complexes with substrate, cofactor, and product. Kinetic analysis of active site mutants demonstrates how a highly conserved aldehyde dehydrogenase active site has been modified in nature to generate activity with a phosphonate substrate. Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation.,Agarwal V, Peck SC, Chen JH, Borisova SA, Chekan JR, van der Donk WA, Nair SK Chem Biol. 2014 Jan 16;21(1):125-35. doi: 10.1016/j.chembiol.2013.11.006. Epub, 2013 Dec 19. PMID:24361046 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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