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| Line 24: |
Line 24: |
| **[[2r6f]] – BstUvrA – ''Bacillus stearothermophilus'' <br /> | | **[[2r6f]] – BstUvrA – ''Bacillus stearothermophilus'' <br /> |
| **[[3ux8]] – GeUvrA - ''Geobacillus''<br /> | | **[[3ux8]] – GeUvrA - ''Geobacillus''<br /> |
| | **[[2vf7]], [[2vf8]] – DrUvrA2 - ''Deinococcus radiodurans''<br /> |
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| *UvrB | | *UvrB |
| Line 38: |
Line 39: |
| *UvrC | | *UvrC |
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| **[[1kft]] – EcUvrC C terminal <br /> | | **[[1kft]], [[1qoj]] – EcUvrC C terminal <br /> |
| | **[[1e52]] – EcUvrC C terminal - NMR<br /> |
| **[[2nrr]], [[2nrt]], [[2nrv]], [[2nrw]], [[2nrx]], [[2nrz]] – TmUvrC C terminal <br /> | | **[[2nrr]], [[2nrt]], [[2nrv]], [[2nrw]], [[2nrx]], [[2nrz]] – TmUvrC C terminal <br /> |
| **[[1ycz]], [[1yd0]], [[1yd1]] – TmUvrC N terminal <br /> | | **[[1ycz]], [[1yd0]], [[1yd1]] – TmUvrC N terminal <br /> |
| Line 44: |
Line 46: |
| **[[1yd6]] – BcUvrC N terminal <br /> | | **[[1yd6]] – BcUvrC N terminal <br /> |
| **[[3c65]] – BstUvrC <br /> | | **[[3c65]] – BstUvrC <br /> |
| | |
| | *UvrD or DNA helicase II |
| | |
| | **[[3lfu]] – EcUvrD <br /> |
| | **[[2is1]], [[2is2]] – EcUvrD (mutant) + DNA<br /> |
| | **[[2is4]] – EcUvrD (mutant) + DNA + ADPNP<br /> |
| | **[[2is6]] – EcUvrD (mutant) + DNA + ADP<br /> |
| | **[[4c2u]] – DrUvrD + DNA<br /> |
| | **[[4c30]] – DrUvrD + DNA + ADPNP<br /> |
| | **[[4c2t]] – DrUvrD (mutant) + DNA + ADPNP<br /> |
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| *UvrA-UvrB | | *UvrA-UvrB |
| Function
UvrABC endonuclease is an E. coli enzyme complex involved in DNA repair. UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one[1].
- UvrA is the protein which locates the DNA damage.
- UvrB is involved in distinguishing damaged from undamaged DNA[2].
- The C-terminal region of UvrC is involved in DNA binding and incisions at the 5'-side of a DNA damage during nucleotide excision repair[3].
- UvrD is DNA helicase II. See Helicase.
For details on the UvrA-UvrB complex see UvrA-UvrB interaction domains.
Structural highlights
UvrA contains several domains: UvrB-binding, DNA-binding and two ATP-binding domains[4].
- ↑ Moolenaar GF, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000 Oct;182(20):5706-14. PMID:11004168
- ↑ Theis K, Skorvaga M, Machius M, Nakagawa N, Van Houten B, Kisker C. The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch. Mutat Res. 2000 Aug 30;460(3-4):277-300. PMID:10946234
- ↑ Moolenaar GF, Uiterkamp RS, Zwijnenburg DA, Goosen N. The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision. Nucleic Acids Res. 1998 Jan 15;26(2):462-8. PMID:9421501
- ↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973
|
3D structures of UvrABC
Updated on 13-October-2017
{"openlevels":0}
- UvrA
- Helicase – TmUvrA + DNA – Thermotoga maritima
- UvrA-UvrB interaction domains – UvrA – Mycobacterium tuberculosis
- 4dfc – EcUvrA + Transcription-repair-coupling factor – Escherichia coli
- 2r6f – BstUvrA – Bacillus stearothermophilus
- 3ux8 – GeUvrA - Geobacillus
- 2vf7, 2vf8 – DrUvrA2 - Deinococcus radiodurans
- UvrB
- 1d2m, 1c4o – UvrB – Thermus thermophilus
- 1d9x – BcUvrB – Bacillus caldotenax
- 1t5l – BcUvrB (mutant)
- 1qoj – EcUvrB C terminal
- 1e52 – EcUvrB C terminal - NMR
- 1d9z – BcUvrB + ATP
- 2fdc – BcUvrB + DNA
- 2d7d, 2nmv, 3v4r – BsUvrB + DNA – Bacillus subtilis
- UvrC
- 1kft, 1qoj – EcUvrC C terminal
- 1e52 – EcUvrC C terminal - NMR
- 2nrr, 2nrt, 2nrv, 2nrw, 2nrx, 2nrz – TmUvrC C terminal
- 1ycz, 1yd0, 1yd1 – TmUvrC N terminal
- 1yd2, 1yd3, 1yd4, 1yd5 – TmUvrC N terminal (mutant)
- 1yd6 – BcUvrC N terminal
- 3c65 – BstUvrC
- UvrD or DNA helicase II
- 3lfu – EcUvrD
- 2is1, 2is2 – EcUvrD (mutant) + DNA
- 2is4 – EcUvrD (mutant) + DNA + ADPNP
- 2is6 – EcUvrD (mutant) + DNA + ADP
- 4c2u – DrUvrD + DNA
- 4c30 – DrUvrD + DNA + ADPNP
- 4c2t – DrUvrD (mutant) + DNA + ADPNP
- UvrA-UvrB
- 3fpn – BstUvrA + UvrB
- 3uwx – GeUvrA + UvrB
References
