4hti

Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin

spinqualitylabelsall models 4hti, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hti is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: PTPRN2, KIAA0387 (HUMAN) Activity: Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PTPR2_HUMAN] Implicated in development of nervous system and pancreatic endocrine cells. Publication Abstract from PubMed Phogrin/IA-2beta and ICA512/IA-2 are two paralogs receptor-type protein-tyrosine phosphatases (RPTP) that localize in secretory granules of various neuroendocrine cells. In pancreatic islet beta-cells, they participate in the regulation of insulin secretion, ensuring proper granulogenesis, and beta-cell proliferation. The role of their cytoplasmic tail has been partially unveiled, while that of their luminal region remains unclear. To advance the understanding of its structure-function relationship, the X-ray structure of the mature ectodomain of phogrin (ME phogrin) at pH 7.4 and 4.6 has been solved at 1.95- and 2.01-A resolution, respectively. Similarly to the ME of ICA512, ME phogrin adopts a ferredoxin-like fold: a sheet of four antiparallel beta-strands packed against two alpha-helices. Sequence conservation among vertebrates, plants and insects suggests that the structural similarity extends to all the receptor family. Crystallized ME phogrin is monomeric, in agreement with solution studies but in striking contrast with the behavior of homodimeric ME ICA512. The structural details that may cause the quaternary structure differences are analyzed. The results provide a basis for building models of the overall orientation and oligomerization state of the receptor in biological membranes. X-ray structure of the mature ectodomain of phogrin.,Noguera ME, Primo ME, Jakoncic J, Poskus E, Solimena M, Ermacora MR J Struct Funct Genomics. 2015 Mar;16(1):1-9. doi: 10.1007/s10969-014-9191-0. Epub, 2014 Nov 26. PMID:25421040 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 3D structures of antibody

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