3wof

Crystal structure of P23-45 gp39 (6-132) bound to Thermus thermophilus RNA polymerase beta-flap domain

spinqualitylabelsall models 3wof, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wof is a 24 chain structure with sequence from Thet8 and Thermus phage p23-45. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3wod, 3woe Gene: rpoB, TTHA1813 (THET8), gp39 (P23p39), P23p39 (Thermus phage P23-45) Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RPOB_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Publication Abstract from PubMed Transcription of DNA to RNA by DNA-dependent RNA polymerase (RNAP) is the first step of gene expression and a major regulation point. Bacteriophages hijack their host's transcription machinery and direct it to serve their needs. The gp39 protein encoded by Thermus thermophilus phage P23-45 binds the host's RNAP and inhibits transcription initiation from its major "-10/-35" class promoters. Phage promoters belonging to the minor "extended -10" class are minimally inhibited. We report the crystal structure of the T. thermophilus RNAP holoenzyme complexed with gp39, which explains the mechanism for RNAP promoter specificity switching. gp39 simultaneously binds to the RNAP beta-flap domain and the C-terminal domain of the sigma subunit (region 4 of the sigma subunit [sigma4]), thus relocating the beta-flap tip and sigma4. The ~45 A displacement of sigma4 is incompatible with its binding to the -35 promoter consensus element, thus accounting for the inhibition of transcription from -10/-35 class promoters. In contrast, this conformational change is compatible with the recognition of extended -10 class promoters. These results provide the structural bases for the conformational modulation of the host's RNAP promoter specificity to switch gene expression toward supporting phage development for gp39 and, potentially, other phage proteins, such as T4 AsiA. Structural basis for promoter specificity switching of RNA polymerase by a phage factor.,Tagami S, Sekine S, Minakhin L, Esyunina D, Akasaka R, Shirouzu M, Kulbachinskiy A, Severinov K, Yokoyama S Genes Dev. 2014 Mar 1;28(5):521-31. doi: 10.1101/gad.233916.113. PMID:24589779 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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