1riq: Difference between revisions
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'''The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase''' | '''The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase''' | ||
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[[Category: Swairjo, M A.]] | [[Category: Swairjo, M A.]] | ||
[[Category: Yang, X L.]] | [[Category: Yang, X L.]] | ||
[[Category: | [[Category: Beta sheet and flanking helice]] | ||
[[Category: | [[Category: Class ii aminoacyl-trna synthetase]] | ||
[[Category: | [[Category: Helix-loop-helix motif]] | ||
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Revision as of 04:32, 3 May 2008
The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase
Overview
Early work on aminoacylation of alanine-specific tRNA (tRNA(Ala)) by alanyl-tRNA synthetase (AlaRS) gave rise to the concept of an early "second genetic code" imbedded in the acceptor stems of tRNAs. A single conserved and position-specific G:U base pair in the tRNA acceptor stem is the key identity determinant. Further understanding has been limited due to lack of a crystal structure of the enzyme. We determined a 2.14 A crystal structure of the 453 amino acid catalytic fragment of Aquifex aeolicus AlaRS. It contains the catalytic domain characteristic of class II synthetases, a helical domain with a hairpin motif critical for acceptor-stem recognition, and a C-terminal domain of a mixed alpha/beta fold. Docking of tRNA(Ala) on AlaRS shows critical contacts with the three domains, consistent with previous mutagenesis and functional data. It also suggests conformational flexibility within the C domain, which might allow for the positional variation of the key G:U base pair seen in some tRNA(Ala)s.
About this Structure
1RIQ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Alanyl-tRNA synthetase crystal structure and design for acceptor-stem recognition., Swairjo MA, Otero FJ, Yang XL, Lovato MA, Skene RJ, McRee DE, Ribas de Pouplana L, Schimmel P, Mol Cell. 2004 Mar 26;13(6):829-41. PMID:15053876 Page seeded by OCA on Sat May 3 07:32:51 2008
