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| ==Your Heading Here (maybe something like 'Structure')== | | ==Anopheles arabiensis Insulin Receptor like Protein== |
| <StructureSection load='Model.itasser.relax.pdb' side='right' caption='Anopheles arabiensis InR kinase domain model' scene='75/750300/Kinase_n_and_c_lobes/1'> | | <StructureSection load='Model.itasser.relax.pdb' side='right' caption='Anopheles arabiensis InR kinase domain model' scene='75/750300/Kinase_n_and_c_lobes/1'> |
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| Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>. | | Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>. |
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| </StructureSection>=='''Anopheles arabiensis Insulin Receptor like Protein''' ==
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| This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1
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| <scene name='75/750300/Inr_atp-cat_loop/3'>This</scene> shows the ATP binding site (purple) and the catalytic loop (green).
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| Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>.
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| These are <scene name='75/750300/Geneticmutations/1'>three ts mutations</scene> found in other RTKs.
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| G1000S (magenta), A1133G (orange), T1221I (teal)
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| And as a <scene name='75/750300/Geneticmutationsspacefilled/1'>spacefilled model</scene>
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| This <scene name='75/750300/T1221_spacefilled/1'>threonine</scene> when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster
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| Using the software TS_pred (a ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>5 best sites</scene> to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red)
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| Using Rosetta_ts_rbf (another ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>top 5</scene> sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow)
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| Both TS_pred and Rosetta_ts_rbf had <scene name='75/750300/Tspredrosetta_spacefilled/1'>L1197</scene> in their top 5 (black)
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| Most but not all of the actual and predicted mutations are <scene name='75/750300/Tspred_rosett_burried_residues/1'>buried</scene>. Note that T1221 and G1000S are not buried.
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| Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>.
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| </StructureSection>
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| </StructureSection> | | </StructureSection> |
