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| The structure of ArgR shows a DNA-binding domain at the acidic N-terminal and a basic C-terminal domain which contains the <scene name='55/554907/Cv/5'>intersubunit interaction sites</scene> and the <scene name='55/554907/Cv/7'>Arg binding site</scene>. Every Arg ligand interacts <scene name='55/554907/Cv/9'>with residues from 3 subunits</scene>.<ref>PMID:8594204</ref> | | The structure of ArgR shows a DNA-binding domain at the acidic N-terminal and a basic C-terminal domain which contains the <scene name='55/554907/Cv/5'>intersubunit interaction sites</scene> and the <scene name='55/554907/Cv/10'>Arg binding site</scene>. Every Arg ligand interacts <scene name='55/554907/Cv/11'>with residues from 3 subunits</scene>.<ref>PMID:8594204</ref> |
| </StructureSection> | | </StructureSection> |
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Revision as of 11:11, 6 January 2019
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Function
Arginine repressor (ArgR) is a prokaryotic repressor which regulates the arginine deiminase pathway. In this pathway, arginine is metabolized to form ammonia, CO2 and ATP. The ArgR releases the expression of the arginine deiminase pathway in the presence of arginine. The genes controlled by ArgR are not found in a single operon. While repressors are usually active as dimers, ArgR is a hexamer and binds to 2 palindromic DNA sites called ARG box in its N terminal domain.[1]
Structural highlights
The structure of ArgR shows a DNA-binding domain at the acidic N-terminal and a basic C-terminal domain which contains the intersubunit interaction sites and the Arg binding site. Every Arg ligand interacts with residues from 3 subunits.[2]
- ↑ Ni J, Sakanyan V, Charlier D, Glansdorff N, Van Duyne GD. Structure of the arginine repressor from Bacillus stearothermophilus. Nat Struct Biol. 1999 May;6(5):427-32. PMID:10331868 doi:https://dx.doi.org/10.1038/8229
- ↑ Van Duyne GD, Ghosh G, Maas WK, Sigler PB. Structure of the oligomerization and L-arginine binding domain of the arginine repressor of Escherichia coli. J Mol Biol. 1996 Feb 23;256(2):377-91. PMID:8594204 doi:https://dx.doi.org/10.1006/jmbi.1996.0093
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3D structures of arginine repressor
Updated on 06-January-2019
{"openlevels":0}
- Arginine repressor
- 1b4a – GsArgR – Geobacillus stearothermophilus
- 1f9n – BsArgR – Bacillus subtilis
- 5cj9 – ArgR – Bacillus halodurans
- 5jvo – ArgR – Corynebacterium pseudotuberculosis
- 3v4g – ArgR – Vibrio vulnificus
- 3ere – MtArgR + DNA – Mycobacterium tuberculosis
- 3fhz, 3laj – MtArgR + DNA + Arg
- 3lap – MtArgR + DNA + canavanine
- Arginine repressor N terminal
- 1aoy – EcArgR N terminal - Escherichia coli - NMR
- 1b4b – BsArgR N terminal
- 2p5l – BsArgR N terminal + DNA
- Arginine repressor C terminal
- 1xxc – EcArgR C terminal
- 2p5k – GsArgR C terminal
- 3bue – MtArgR C terminal
- 1xxa, 1xxb – EcArgR C terminal + Arg
- 2p5m – BsArgR C terminal (mutant) + Arg
- 2zfz, 3cag – MtArgR C terminal + Arg
References
