1gvg: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1gvg. | [[Image:1gvg.jpg|left|200px]]<br /><applet load="1gvg" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1gvg" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1gvg, resolution 1.54Å" /> | caption="1gvg, resolution 1.54Å" /> | ||
'''CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE'''<br /> | '''CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1GVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with FE, SO4, NO, AKG and PCX as [http://en.wikipedia.org/wiki/ligands ligands]. | 1GVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with FE, SO4, NO, AKG and PCX as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Nmo Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA]. | ||
==Reference== | ==Reference== | ||
| Line 33: | Line 32: | ||
[[Category: trifunctional enzyme]] | [[Category: trifunctional enzyme]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:39:29 2007'' | ||
Revision as of 13:29, 18 December 2007
|
CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE
Overview
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of, CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were, exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to, exposure with NO, the active site Fe(II) is octahedrally coordinated by a, water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the, side-chains of an aspartyl and two histidinyl residues. NO binds to the, position previously occupied by the 2OG 1-carboxylate concomitant with, rearrangement of the latter to the position previously occupied by the, displaced water.
About this Structure
1GVG is a Single protein structure of sequence from Streptomyces clavuligerus with FE, SO4, NO, AKG and PCX as ligands. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399
Page seeded by OCA on Tue Dec 18 15:39:29 2007
