5kwd: Difference between revisions

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'''Unreleased structure'''


The entry 5kwd is ON HOLD  until Paper Publication
==Computationally Designed Symmetric Homotetramer==
<StructureSection load='5kwd' size='340' side='right' caption='[[5kwd]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5kwd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KWD FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kwd OCA], [http://pdbe.org/5kwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kwd RCSB], [http://www.ebi.ac.uk/pdbsum/5kwd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kwd ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Self-assembling cyclic protein homo-oligomers play important roles in biology, and the ability to generate custom homo-oligomeric structures could enable new approaches to probe biological function. Here we report a general approach to design cyclic homo-oligomers that employs a new residue-pair-transform method to assess the designability of a protein-protein interface. This method is sufficiently rapid to enable the systematic enumeration of cyclically docked arrangements of a monomer followed by sequence design of the newly formed interfaces. We use this method to design interfaces onto idealized repeat proteins that direct their assembly into complexes that possess cyclic symmetry. Of 96 designs that were characterized experimentally, 21 were found to form stable monodisperse homo-oligomers in solution, and 15 (four homodimers, six homotrimers, six homotetramers and one homopentamer) had solution small-angle X-ray scattering data consistent with the design models. X-ray crystal structures were obtained for five of the designs and each is very close to their corresponding computational model.


Authors: Fallas, J.A., Sankaran, B., Zwart, P., Baker, D.
Computational design of self-assembling cyclic protein homo-oligomers.,Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D Nat Chem. 2017 Apr;9(4):353-360. doi: 10.1038/nchem.2673. Epub 2016 Dec 5. PMID:28338692<ref>PMID:28338692</ref>


Description: Computationally Designed Symmetric Homotetramer
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5kwd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Baker, D]]
[[Category: Baker, D]]
[[Category: Fallas, J.A]]
[[Category: Fallas, J A]]
[[Category: Sankaran, B]]
[[Category: Zwart, P]]
[[Category: Zwart, P]]
[[Category: Sankaran, B]]
[[Category: Ankyrin]]
[[Category: De novo protein]]
[[Category: Designed protein]]
[[Category: Homooligomer]]
[[Category: Repeat protein]]
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