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'''Unreleased structure'''


The entry 5ljf is ON HOLD  until Paper Publication
==Crystal structure of the endo-1,4-glucanase RBcel1 E135A with cellotriose==
<StructureSection load='5ljf' size='340' side='right' caption='[[5ljf]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ljf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CT3:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CT3</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ee9|4ee9]], [[4m24|4m24]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ljf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljf OCA], [http://pdbe.org/5ljf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljf RCSB], [http://www.ebi.ac.uk/pdbsum/5ljf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljf ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.


Authors: Dutoit, R., Collet, L., Galleni, M., Bauvois, C.
Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.,Berlemont R, Delsaute M, Pipers D, D'Amico S, Feller G, Galleni M, Power P ISME J. 2009 Sep;3(9):1070-81. doi: 10.1038/ismej.2009.48. Epub 2009 May 21. PMID:19458657<ref>PMID:19458657</ref>


Description: Crystal structure of the endo-1,4-glucanase RBcel1 E135A with cellotriose
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5ljf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cellulase]]
[[Category: Uncultivated bacterium]]
[[Category: Bauvois, C]]
[[Category: Collet, L]]
[[Category: Collet, L]]
[[Category: Dutoit, R]]
[[Category: Galleni, M]]
[[Category: Galleni, M]]
[[Category: Dutoit, R]]
[[Category: 4-endoglucanase]]
[[Category: Bauvois, C]]
[[Category: Beta-1]]
[[Category: Glycosyl hydrolase family 5]]
[[Category: Hydrolase]]
[[Category: Tim barrel]]
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