5mr6: Difference between revisions

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'''Unreleased structure'''


The entry 5mr6 is ON HOLD  until Paper Publication
==XiaF from Streptomyces sp. in complex with FADH2 and Glycerol==
<StructureSection load='5mr6' size='340' side='right' caption='[[5mr6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5mr6]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MR6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jbr|2jbr]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xiaF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mr6 OCA], [http://pdbe.org/5mr6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mr6 RCSB], [http://www.ebi.ac.uk/pdbsum/5mr6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mr6 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Terpenoid natural products comprise a wide range of molecular architectures that typically result from C-C bond formations catalysed by classical type I/II terpene cyclases. However, the molecular diversity of biologically active terpenoids is substantially increased by fully unrelated, non-canonical terpenoid cyclases. Their evolutionary origin has remained enigmatic. Here we report the in vitro reconstitution of an unusual flavin-dependent bacterial indoloterpenoid cyclase, XiaF, together with a designated flavoenzyme-reductase (XiaP) that mediates a key step in xiamycin biosynthesis. The crystal structure of XiaF with bound FADH2 (at 2.4 A resolution) and phylogenetic analyses reveal that XiaF is, surprisingly, most closely related to xenobiotic-degrading enzymes. Biotransformation assays show that XiaF is a designated indole hydroxylase that can be used for the production of indigo and indirubin. We unveil a cryptic hydroxylation step that sets the basis for terpenoid cyclization and suggest that the cyclase has evolved from xenobiotics detoxification enzymes.


Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C.
Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification.,Kugel S, Baunach M, Baer P, Ishida-Ito M, Sundaram S, Xu Z, Groll M, Hertweck C Nat Commun. 2017 Jun 15;8:15804. doi: 10.1038/ncomms15804. PMID:28643772<ref>PMID:28643772</ref>


Description: XiaF from Streptomyces sp. in complex with FADH2 and Glycerol
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5mr6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chainia sp]]
[[Category: Baer, P]]
[[Category: Baunach, M]]
[[Category: Groll, M]]
[[Category: Hertweck, C]]
[[Category: Hertweck, C]]
[[Category: Ishida-Ito, M]]
[[Category: Kugel, S]]
[[Category: Sundaram, S]]
[[Category: Sundaram, S]]
[[Category: Kugel, S]]
[[Category: Baunach, M]]
[[Category: Ishida-Ito, M]]
[[Category: Xu, Z]]
[[Category: Xu, Z]]
[[Category: Groll, M]]
[[Category: Detoxification]]
[[Category: Baer, P]]
[[Category: Indigo]]
[[Category: Indirubin]]
[[Category: Non-canonical flavin-dependent terpenoid cyclase]]
[[Category: Oxidoreductase]]
[[Category: Xenobiotics-degrading enzyme]]
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