5n00: Difference between revisions
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==Crystal structure of the decarboxylase AibA/AibB C56A variant== | |||
<StructureSection load='5n00' size='340' side='right' caption='[[5n00]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5n00]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N00 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n00 OCA], [http://pdbe.org/5n00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n00 RCSB], [http://www.ebi.ac.uk/pdbsum/5n00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n00 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Isovaleryl coenzyme A (IV-CoA) is an important precursor for iso-fatty acids and lipids. It acts in the development of myxobacteria, which can produce this compound from acetyl-CoA through alternative IV-CoA biosynthesis (aib). A central reaction of aib is catalyzed by AibA/AibB, which acts as a cofactor-free decarboxylase despite belonging to the family of CoA-transferases. We developed an efficient expression system for AibA/AibB that allowed the determination of high-resolution crystal structures in complex with different ligands. Through mutational studies, we show that an active-site cysteine previously proposed to be involved in decarboxylation is not required for activity. Instead, AibA/AibB seems to induce an intramolecular decarboxylation by binding its substrate in a hydrophobic cavity and forcing it into a bent conformation. Our study opens opportunities for synthetic biology studies, since AibA/AibB may be suitable for the production of isobutene, a precursor of biofuels and chemicals. | |||
AibA/AibB Induces an Intramolecular Decarboxylation in Isovalerate Biosynthesis by Myxococcus xanthus.,Bock T, Luxenburger E, Hoffmann J, Schutza V, Feiler C, Muller R, Blankenfeldt W Angew Chem Int Ed Engl. 2017 Aug 7;56(33):9986-9989. doi: 10.1002/anie.201701992., Epub 2017 May 16. PMID:28508504<ref>PMID:28508504</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 5n00" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Blankenfeldt, W]] | |||
[[Category: Bock, T]] | [[Category: Bock, T]] | ||
[[Category: Feiler, C]] | [[Category: Feiler, C]] | ||
[[Category: Hoffmann, J]] | [[Category: Hoffmann, J]] | ||
[[Category: Luxenburger, E]] | |||
[[Category: Mueller, R]] | |||
[[Category: Schuetza, V]] | |||
[[Category: Coa transferase like fold]] | |||
[[Category: Decarboxylase]] | |||
[[Category: Lyase]] | |||
Revision as of 09:11, 9 August 2017
Crystal structure of the decarboxylase AibA/AibB C56A variant
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