5nbw: Difference between revisions

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'''Unreleased structure'''


The entry 5nbw is ON HOLD
==Crystal structure of the Fab fragment 22F12 in complex with 3-hydroxybenzo[a]pyrene==
<StructureSection load='5nbw' size='340' side='right' caption='[[5nbw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nbw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NBW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8SK:benzo[a]pyren-3-ol'>8SK</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbw OCA], [http://pdbe.org/5nbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nbw RCSB], [http://www.ebi.ac.uk/pdbsum/5nbw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Benzo[a]pyrene, which is produced during the incomplete combustion of organic material, is an abundant noxious pollutant because of its carcinogenic metabolic degradation products. The high-affinity (KD approximately 3 nm) monoclonal antibody 22F12 allows facile bioanalytical quantification of benzo[a]pyrene even in complex matrices. We report the functional and X-ray crystallographic analysis of 22F12 in complex with 3-hydroxybenzo[a]pyrene after cloning of the V-genes and production as a recombinant Fab fragment. The polycyclic aromatic hydrocarbon is bound in a deep pocket between the light and heavy chains, surrounded mainly by aromatic and aliphatic amino acid side chains. Interestingly, the hapten-antibody interface is less densely packed than expected and reveals polar, H-bond-like interactions with the polycyclic aromatic pi-electron system, which may allow the antibody to maintain a large, predominantly hydrophobic binding site in an aqueous environment while providing sufficient complementarity to its ligand.


Authors: Skerra, A., Eichinger, A.
Tight Molecular Recognition of Benzo[a]pyrene by a High-Affinity Antibody.,Eichinger A, Neumaier I, Pschenitza M, Niessner R, Knopp D, Skerra A Angew Chem Int Ed Engl. 2017 Aug 21;56(35):10592-10597. doi:, 10.1002/anie.201703893. Epub 2017 Jul 21. PMID:28603847<ref>PMID:28603847</ref>


Description: Crystal structure of the Fab fragment 22F12 in complex with 3-hydroxybenzo[a]pyrene
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5nbw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Eichinger, A]]
[[Category: Eichinger, A]]
[[Category: Skerra, A]]
[[Category: Skerra, A]]
[[Category: Antibody]]
[[Category: Fab fragment]]
[[Category: Hapten]]
[[Category: Immune system]]
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