5um8: Difference between revisions
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==Crystal structure of HIV-1 envelope trimer 16055 NFL TF CC T(569)G in complex with Fabs 35022 and PGT124== | |||
<StructureSection load='5um8' size='340' side='right' caption='[[5um8]], [[Resolution|resolution]] 3.94Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5um8]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UM8 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5um8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5um8 OCA], [http://pdbe.org/5um8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5um8 RCSB], [http://www.ebi.ac.uk/pdbsum/5um8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5um8 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies. However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability. Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state. We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 A. Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans. Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development. | |||
Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.,Guenaga J, Garces F, de Val N, Stanfield RL, Dubrovskaya V, Higgins B, Carrette B, Ward AB, Wilson IA, Wyatt RT Immunity. 2017 May 16;46(5):792-803.e3. doi: 10.1016/j.immuni.2017.04.014. PMID:28514686<ref>PMID:28514686</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5um8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Garces, F]] | |||
[[Category: Stanfield, R L]] | |||
[[Category: Wilson, I A]] | |||
[[Category: Hiv-1 antibody glycoprotein]] | |||
[[Category: Viral protein-immune system complex]] | |||
Revision as of 13:40, 24 May 2017
Crystal structure of HIV-1 envelope trimer 16055 NFL TF CC T(569)G in complex with Fabs 35022 and PGT124
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