1t1c: Difference between revisions
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'''Late intermediate IL3 from time-resolved crystallography of the E46Q mutant of PYP''' | '''Late intermediate IL3 from time-resolved crystallography of the E46Q mutant of PYP''' | ||
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[[Category: Schmidt, M.]] | [[Category: Schmidt, M.]] | ||
[[Category: Srajer, V.]] | [[Category: Srajer, V.]] | ||
[[Category: | [[Category: Pas domain]] | ||
[[Category: | [[Category: Photoactive yellow protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:23:01 2008'' | |||
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Revision as of 06:23, 3 May 2008
Late intermediate IL3 from time-resolved crystallography of the E46Q mutant of PYP
Overview
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
About this Structure
1T1C is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:15642261 Page seeded by OCA on Sat May 3 09:23:01 2008
