5gqm

Crystal structure of in cellulo Wild Type Cypovirus Polyhedra

spinqualitylabelsall models 5gqm, resolution 1.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5gqm is a 1 chain structure with sequence from Bmcpv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, CL, CTP, GTP, MG NonStd Res: ACE Related: 5gqi, 5gqj, 5gqk, 5gql, 5gqn Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PYHD_CPVBM] Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae. Publication Abstract from PubMed Crystalline porous materials have been investigated for development of important applications in molecular storage, separations, and catalysis. The potential of protein crystals is increasing as they become better understood. Protein crystals have been regarded as porous materials because they present highly ordered 3D arrangements of protein molecules with high porosity and wide range of pore sizes. However, it remains difficult to functionalize protein crystals in living cells. Here, we report that polyhedra, a natural crystalline protein assembly of polyhedrin monomer (PhM) produced in insect cells infected by cypovirus, can be engineered to extend porous networks by deleting selected amino acid residues located on the intermolecular contact region of PhM. The adsorption rates and quantities of fluorescent dyes stored within the mutant crystals are increased relative to those of the wild-type polyhedra crystal (WTPhC) under both in vitro and in vivo conditions. These results provide a strategy for designing self-assembled protein materials with applications in molecular recognition and storage of exogenous substances in living cell as well as an entry point for development of bioorthogonal chemistry and in vivo crystal structure analysis. Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells.,Abe S, Tabe H, Ijiri H, Yamashita K, Hirata K, Atsumi K, Shimoi T, Akai M, Mori H, Kitagawa S, Ueno T ACS Nano. 2017 Feb 9. doi: 10.1021/acsnano.6b06099. PMID:28094987 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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