5hr1

Crystal structure of thioredoxin L107A mutant

spinqualitylabelsall models 5hr1, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hr1 is a 7 chain structure with sequence from Eco57. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU Related: 5hr3, 5hr2, 5hr0 Gene: trxA, Z5291, ECs4714 (ECO57) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [THIO_ECO57] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Publication Abstract from PubMed Thioredoxin is a ubiquitous small protein that catalyzes redox reactions of protein thiols. Additionally, thioredoxin from E. coli (EcTRX) is a widely-used model for structure-function studies. In a previous paper, we characterized several single-point mutants of the C-terminal helix (CTH) that alter global stability of EcTRX. However, spectroscopic signatures and enzymatic activity for some of these mutants were found essentially unaffected. A comprehensive structural characterization at the atomic level of these near-invariant mutants can provide detailed information about structural variability of EcTRX. We address this point through the determination of the crystal structures of four point-mutants, whose mutations occurs within or near the CTH, namely L94A, E101G, N106A and L107A. These structures are mostly unaffected compared with the wild-type variant. Notably, the E101G mutant presents a large region with two alternative traces for the backbone of the same chain. It represents a significant shift in backbone positions. Enzymatic activity measurements and conformational dynamics studies monitored by NMR and molecular dynamic simulations show that E101G mutation results in a small effect in the structural features of the protein. We hypothesize that these alternative conformations represent samples of the native-state ensemble of EcTRX, specifically the magnitude and location of conformational heterogeneity. Structural variability of E. coli thioredoxin captured in the crystal structures of single-point mutants.,Noguera ME, Vazquez DS, Ferrer-Sueta G, Agudelo WA, Howard E, Rasia RM, Manta B, Cousido-Siah A, Mitschler A, Podjarny A, Santos J Sci Rep. 2017 Feb 9;7:42343. doi: 10.1038/srep42343. PMID:28181556 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Thioredoxin 3D structures

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