4x35

A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering

spinqualitylabelsall models 4x35, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4x35 is a 1 chain structure with sequence from Operophtera brumata cypovirus 18. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, CL, GTP, MG NonStd Res: ACE, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed At low emittance synchrotron sources it has become possible to perform structure determinations from the measurement of multiple microcrystals which were previously considered too small for diffraction experiments. Conventional mounting techniques do not fulfill the requirements of these new experiments. They significantly contribute to background scattering and it is difficult to locate the crystals, making them incompatible with automated serial crystallography. We have developed a micro-fabricated sample holder from single crystalline silicon with micropores, which carries up to thousands of crystals and significantly reduces the background scattering level. For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subsequently soaked off through the micropores. Crystals larger than the pore size are retained and arrange themselves according to the micropore pattern. Using our chip we were able to collect 1.5 A high resolution diffraction data from protein microcrystals with sizes of 4 micrometers and smaller. A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering.,Roedig P, Vartiainen I, Duman R, Panneerselvam S, Stube N, Lorbeer O, Warmer M, Sutton G, Stuart DI, Weckert E, David C, Wagner A, Meents A Sci Rep. 2015 May 29;5:10451. doi: 10.1038/srep10451. PMID:26022615 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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