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== Biological Function == | == Biological Function == | ||
Czr A is a transcriptional repressor protein responsible for the regulation of the | Czr A is a transcriptional repressor protein responsible for the regulation of the Chromosome Determined Zinc Responsible (Czr) operon. The Czr operon contains genes for the proteins Czr A and Czr B. Czr B is a Zinc transport protein which moves Zinc out of a cell. Czr A regulates this process by controlling the degree to which Czr B is expressed. When relatively low amounts of zinc are present in the cell Czr A will bind to DNA, inhibiting expression of Czr B, enabling the cell to retain Zinc. When high amounts of Zinc 2+ are present in the cell, each Czr A molecule binds two Zn 2+ ions, and forcing a conformational change. The zinc binding conformation of Czr A is not capable of binding to DNA, as the new conformation disrupts the DNA binding pocket. Conversely, the DNA binding conformation of Czr A does not allow for the binding of either Zn 2+ atom. | ||
== Structural Overview == | == Structural Overview == | ||
===DNA Binding === | ===DNA Binding === | ||
Revision as of 13:20, 14 March 2017
| This Sandbox is Reserved from 02/09/2015, through 05/31/2016 for use in the course "CH462: Biochemistry 2" taught by Geoffrey C. Hoops at the Butler University. This reservation includes Sandbox Reserved 1051 through Sandbox Reserved 1080. |
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Czr A Staphylococcus aureus
<StructureSection load='2kjc' size='340' side='right' caption='Caption for this structure' scene=>
This is a default text for your page '. Click above on edit this page' to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue.
Biological Function
Czr A is a transcriptional repressor protein responsible for the regulation of the Chromosome Determined Zinc Responsible (Czr) operon. The Czr operon contains genes for the proteins Czr A and Czr B. Czr B is a Zinc transport protein which moves Zinc out of a cell. Czr A regulates this process by controlling the degree to which Czr B is expressed. When relatively low amounts of zinc are present in the cell Czr A will bind to DNA, inhibiting expression of Czr B, enabling the cell to retain Zinc. When high amounts of Zinc 2+ are present in the cell, each Czr A molecule binds two Zn 2+ ions, and forcing a conformational change. The zinc binding conformation of Czr A is not capable of binding to DNA, as the new conformation disrupts the DNA binding pocket. Conversely, the DNA binding conformation of Czr A does not allow for the binding of either Zn 2+ atom.
Structural Overview
DNA Binding
Zinc Binding
Residues involved in Zn 2+ binding
Zinc Ligand(s)
This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:https://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
