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'''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION''' | '''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION''' | ||
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[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: | [[Category: Cryo-electron microscopy]] | ||
[[Category: | [[Category: Cryo-em]] | ||
[[Category: | [[Category: Dengue virus]] | ||
[[Category: | [[Category: Flaviviridae]] | ||
[[Category: | [[Category: Flavivirus]] | ||
[[Category: | [[Category: Glycoprotein e]] | ||
[[Category: | [[Category: Icosahedral virus]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:57:03 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 06:57, 3 May 2008
COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION
Overview
Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses.
About this Structure
1THD is a Single protein structure of sequence from Dengue virus 2. Full crystallographic information is available from OCA.
Reference
Conformational changes of the flavivirus E glycoprotein., Zhang Y, Zhang W, Ogata S, Clements D, Strauss JH, Baker TS, Kuhn RJ, Rossmann MG, Structure. 2004 Sep;12(9):1607-18. PMID:15341726 Page seeded by OCA on Sat May 3 09:57:03 2008
