1h0d: Difference between revisions
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==Overview== | ==Overview== | ||
The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use. | The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use. | ||
==Disease== | |||
Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=105850 105850]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:11:10 2007'' | ||
