5x31: Difference between revisions
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==Pseudoazurin from Alcaligenes faecalis (space group P65)== | |||
<StructureSection load='5x31' size='340' side='right' caption='[[5x31]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5x31]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X31 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x31 OCA], [http://pdbe.org/5x31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x31 RCSB], [http://www.ebi.ac.uk/pdbsum/5x31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x31 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pseudoazurin from the denitrifying bacterium Alcaligenes faecalis (AfPAz) is a blue copper protein and functions as an electron donor to copper-containing nitrite reductase (CuNIR). Conventionally, AfPAz has been crystallized using highly concentrated ammonium sulfate as a precipitant. Here, a needle-like crystal of AfPAz grown in a solution containing a macromolecular precipitant, polyethylene glycol 8000 (PEG 8000), is reported. The crystal belonged to space group P61, with unit-cell parameters a = b = 68.7, c = 94.2 A. The structure has been determined and refined at 2.6 A resolution. The asymmetric unit contained two AfPAz molecules contacting each other on negatively charged surfaces. The molecular packing of the crystal showed a right-handed double-helical arrangement of AfPAz molecules and hence of blue copper sites. This structure provides insight into the excluded-volume effect of PEG and the manner of assembly of AfPAz. | |||
New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.,Fukuda Y, Mizohata E, Inoue T Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):159-166. doi:, 10.1107/S2053230X17002631. Epub 2017 Feb 28. PMID:28291752<ref>PMID:28291752</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5x31" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fukuda, Y]] | |||
[[Category: Inoue, T]] | |||
[[Category: Mizohata, E]] | [[Category: Mizohata, E]] | ||
[[Category: | [[Category: Copper]] | ||
[[Category: | [[Category: Cupredoxin]] | ||
[[Category: Electron transfer]] | |||
[[Category: Electron transport]] | |||
[[Category: Pseudoazurin]] | |||
