5a6w

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with the HMA domain of Pikp1 from rice (Oryza sativa)

spinqualitylabelsall models 5a6w, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5a6w is a 3 chain structure with sequence from magnaporthe_oryzae Bpi 841383 magnaporthe oryzae and Orysa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, SO4 Related: 5a6p Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Plants have evolved intracellular immune receptors to detect pathogen proteins known as effectors. How these immune receptors detect effectors remains poorly understood. Here we describe the structural basis for direct recognition of AVR-Pik, an effector from the rice blast pathogen, by the rice intracellular NLR immune receptor Pik. AVR-PikD binds a dimer of the Pikp-1 HMA integrated domain with nanomolar affinity. The crystal structure of the Pikp-HMA/AVR-PikD complex enabled design of mutations to alter protein interaction in yeast and in vitro, and perturb effector-mediated response both in a rice cultivar containing Pikp and upon expression of AVR-PikD and Pikp in the model plant Nicotiana benthamiana. These data reveal the molecular details of a recognition event, mediated by a novel integrated domain in an NLR, which initiates a plant immune response and resistance to rice blast disease. Such studies underpin novel opportunities for engineering disease resistance to plant pathogens in staple food crops. Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.,Maqbool A, Saitoh H, Franceschetti M, Stevenson C, Uemura A, Kanzaki H, Kamoun S, Terauchi R, Banfield MJ Elife. 2015 Aug 25;4. doi: 10.7554/eLife.08709. PMID:26304198 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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