1tv3: Difference between revisions
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'''Crystal structure of the N-methyl-hydroxylamine MtmB complex''' | '''Crystal structure of the N-methyl-hydroxylamine MtmB complex''' | ||
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[[Category: Soares, J A.]] | [[Category: Soares, J A.]] | ||
[[Category: Zhao, G.]] | [[Category: Zhao, G.]] | ||
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:24:14 2008'' | |||
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Revision as of 07:24, 3 May 2008
Crystal structure of the N-methyl-hydroxylamine MtmB complex
Overview
L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
About this Structure
1TV3 is a Single protein structure of sequence from Methanosarcina barkeri. Full crystallographic information is available from OCA.
Reference
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid., Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK, Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192 Page seeded by OCA on Sat May 3 10:24:14 2008
