5ukz: Difference between revisions
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==NMR Solution structure of chemically synthesized antilisterial Pediocin PA-1 M31L analog.== | |||
<StructureSection load='5ukz' size='340' side='right' caption='[[5ukz]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5ukz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UKZ FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ukz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ukz OCA], [http://pdbe.org/5ukz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ukz RCSB], [http://www.ebi.ac.uk/pdbsum/5ukz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ukz ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/PPA1_PEDAC PPA1_PEDAC]] Bactericidal activity (effective inhibitor of L.monocytogenes). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The antimicrobial peptide pediocin PA-1 is a class IIa bacteriocin that inhibits several clinically relevant pathogens including Listeria spp. Here we report the synthesis and characterization of whole pediocin PA-1 and novel analogs thereof using a combination of solid- and solution-phase strategies to overcome difficulties due to instability and undesired reactions. Pediocin PA-1 thus synthesized was a potent inhibitor of Listeria monocytogenes (MIC = 6.8 nM), similar to the bacteriocin produced naturally by Pediococcus acidilactici. Of particular interest is that linear analogs lacking both of the disulfide bridges characterizing pediocin PA-1 were as potent. One linear analog was also a strong inhibitor of Clostridium perfringens, another important food-borne pathogen. These results are discussed in light of conformational information derived from circular dichroism, solution NMR spectroscopy and structure-activity relationship studies. | |||
Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof.,Bedard F, Hammami R, Zirah S, Rebuffat S, Fliss I, Biron E Sci Rep. 2018 Jun 13;8(1):9029. doi: 10.1038/s41598-018-27225-3. PMID:29899567<ref>PMID:29899567</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5ukz" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bedard, F]] | |||
[[Category: Biron, E]] | |||
[[Category: Fliss, I]] | |||
[[Category: Hammami, R]] | |||
[[Category: Rebuffat, S]] | |||
[[Category: Zirah, S]] | |||
[[Category: Antilisterial]] | |||
[[Category: Antimicrobial protein]] | |||
[[Category: Bacteriocin]] | |||
[[Category: Chemical synthesis]] | |||
[[Category: Pediocin pa-1]] | |||
[[Category: Pediococcus acidilactici ul5]] | |||
