5vco: Difference between revisions

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vco OCA], [http://pdbe.org/5vco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vco RCSB], [http://www.ebi.ac.uk/pdbsum/5vco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vco ProSAT]</span></td></tr>

Der p 1 is a major allergen from the house dust mite, Dermatophagoides pteronyssinus, that belongs to the papain-like cysteine protease family. To investigate the antigenic determinants of Der p 1, we determined two crystal structures of Der p 1 in complex with the Fab fragments of mAbs 5H8 or 10B9. Epitopes for these two Der p 1-specific Abs are located in different, nonoverlapping parts of the Der p 1 molecule. Nevertheless, surface area and identity of the amino acid residues involved in hydrogen bonds between allergen and Ab are similar. The epitope for mAb 10B9 only showed a partial overlap with the previously reported epitope for mAb 4C1, a cross-reactive mAb that binds Der p 1 and its homolog Der f 1 from Dermatophagoides farinae. Upon binding to Der p 1, the Fab fragment of mAb 10B9 was found to form a very rare alpha helix in its third CDR of the H chain. To provide an overview of the surface properties of the interfaces formed by the complexes of Der p 1-10B9 and Der p 1-5H8, along with the complexes of 4C1 with Der p 1 and Der f 1, a broad analysis of the surfaces and hydrogen bonds of all complexes of Fab-protein or Fab-peptide was performed. This work provides detailed insight into the cross-reactive and specific allergen-Ab interactions in group 1 mite allergens. The surface data of Fab-protein and Fab-peptide interfaces can be used in the design of conformational epitopes with reduced Ab binding for immunotherapy.

Structural Analysis of Der p 1-Antibody Complexes and Comparison with Complexes of Proteins or Peptides with Monoclonal Antibodies.,Osinski T, Pomes A, Majorek KA, Glesner J, Offermann LR, Vailes LD, Chapman MD, Minor W, Chruszcz M J Immunol. 2015 Jul 1;195(1):307-16. doi: 10.4049/jimmunol.1402199. Epub 2015 May, 29. PMID:26026055<ref>PMID:26026055</ref>

[[Category: Chapman, M D]]

[[Category: Chruszcz, M]]

[[Category: Glesner, J]]

[[Category: Majorek, K A]]

[[Category: Offermann, L R]]

[[Category: Pomes, A]]

[[Category: Vailes, L D]]

[[Category: Allergen]]

[[Category: Antibody]]

[[Category: Immune system]]