Sandbox Reserved 1069: Difference between revisions
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[[Image:FRET.png|200px|left|thumb| CTD of both monomers were measured to be closer together when saturated with Zn<sup>2+</sup>]] | [[Image:FRET.png|200px|left|thumb| CTD of both monomers were measured to be closer together when saturated with Zn<sup>2+</sup>]] | ||
In contrast the main purpose of conformation change in the CTD is to stabilize the YiiP dimer and to act as a Zn<sup>2+</sup> sensor. | In contrast the main purpose of conformation change in the CTD is to stabilize the YiiP dimer and to act as a Zn<sup>2+</sup> sensor. | ||
This is possible because of the flexible loop that links the TMD and the CTD. This loop harbors the | This is possible because of the flexible loop that links the TMD and the CTD. This loop harbors the <scene name='75/756372/Bestsaltbridgetransparent/1'>salt bridge</scene> which serves as a hinge that allows movement of the CTD. Using [https://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] to measure the distance between the CTD of each monomer fluorescence quenching was observed as the concentration Zn<sup>2+</sup> increased, which supports that idea that Zn<sup>2+</sup> induces a stabilizing conformation change in the CTD.<ref>PMID:19749753</ref> | ||
