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'''Crystal Structure of Pseudomonas Avirulence Protein AvrPphB''' | '''Crystal Structure of Pseudomonas Avirulence Protein AvrPphB''' | ||
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[[Category: Xu, Z.]] | [[Category: Xu, Z.]] | ||
[[Category: Zhu, M.]] | [[Category: Zhu, M.]] | ||
[[Category: | [[Category: Avirulence]] | ||
[[Category: | [[Category: Avrpph3]] | ||
[[Category: | [[Category: Avrpphb]] | ||
[[Category: | [[Category: Hypersensitive response]] | ||
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Revision as of 08:21, 3 May 2008
Crystal Structure of Pseudomonas Avirulence Protein AvrPphB
Overview
AvrPphB is an avirulence (Avr) protein from the plant pathogen Pseudomonas syringae that can trigger a disease-resistance response in a number of host plants including Arabidopsis. AvrPphB belongs to a novel family of cysteine proteases with the charter member of this family being the Yersinia effector protein YopT. AvrPphB has a very stringent substrate specificity, catalyzing a single proteolytic cleavage in the Arabidopsis serine/threonine kinase PBS1. We have determined the crystal structure of AvrPphB by x-ray crystallography at 1.35-A resolution. The structure is composed of a central antiparallel beta-sheet, with alpha-helices packing on both sides of the sheet to form a two-lobe structure. The core of this structure resembles the papain-like cysteine proteases. The similarity includes the AvrPphB active site catalytic triad of Cys-98, His-212, and Asp-227 and the oxyanion hole residue Asn-93. Based on analogy with inhibitor complexes of the papain-like proteases, we propose a model for the substrate-binding mechanism of AvrPphB. A deep and positively charged pocket (S2) and a neighboring shallow surface (S3) likely bind to aspartic acid and glycine residues in the substrate located two (P2) and three (P3) residues N terminal to the cleavage site, respectively. Further implications about the specificity of plant pathogen recognition are also discussed.
About this Structure
1UKF is a Single protein structure of sequence from Pseudomonas syringae pv. phaseolicola. Full crystallographic information is available from OCA.
Reference
The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site., Zhu M, Shao F, Innes RW, Dixon JE, Xu Z, Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):302-7. Epub 2003 Dec 23. PMID:14694194 Page seeded by OCA on Sat May 3 11:21:00 2008
