Sandbox Reserved 1072: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 28: | Line 28: | ||
===CZB Domain=== | ===CZB Domain=== | ||
The <scene name='69/694239/Zb_domain_residues_19-90/4'>CZB Domain</scene>, residues 19-90, is responsible for regulating the function of DgcZ. The domain contains the allosteric binding site of the enzyme with cooperative binding. Four residues bind zinc with a high affinity even at 10<sup>- | The <scene name='69/694239/Zb_domain_residues_19-90/4'>CZB Domain</scene>, residues 19-90, is responsible for regulating the function of DgcZ. The domain contains the allosteric binding site of the enzyme with cooperative binding. Four residues bind zinc with a high affinity even at 10<sup>-16</sup>M concentrations. Due to the tightness of Zinc binding, the enzyme has not yet been crystallized in the active conformation without the presence of Zinc metal inhibitor. When zinc is bound, DgcZ activity is limited<sup>[1]</sup>. Two Zinc binding sites are located on the CZB domain. | ||
[[Image:Zinc binding site labels.jpg|250 px|left|thumb|Zn<sup>+2</sup> Coordination to amino acid residues on three of the four 𝝰 helices of DgcZ]] | [[Image:Zinc binding site labels.jpg|250 px|left|thumb|Zn<sup>+2</sup> Coordination to amino acid residues on three of the four 𝝰 helices of DgcZ]] | ||
