Sandbox Reserved 1063: Difference between revisions

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==='''Structural Overview'''===

One of the two functional domains of AdcR is <scene name='69/694230/Dimerization_domain/3'> dimerization domain</scene>. This domain connects and stabilizes the two pseudosymmetric dimers and is composed of the <scene name='69/694230/Alpha_1/1'>α1 helix</scene>, the C-terminus of the <scene name='69/694230/Alpha_five/1'>α5 helix</scene> , and the <scene name='69/694230/Alpha_6/1'>α6 helix</scene>. This domain is connected to the DNA binding domain by the long α5 helix. The DNA binding domain stabilizes the major and minor groove of DNA via the winged helix-turn-helix motif. Binding of Zinc allows AdcR to bind DNA and activate the transcription of high-affinity Zinc specific uptake transporters. The binding of Zinc to the <scene name='69/694230/2_binding_sites/2'>Zinc binding pocket</scene> induces a conformational change that allows for a hydrogen bond network between helices of the binding domain. It is believed that this hydrogen bond network is the allosteric activator needed to expose residues that bind the bases along the major groove of the DNA <ref name="guerra">PMID:22085181</ref>. The binding sites are found adjacent to the DNA binding domain.

One of the two functional domains of AdcR is <scene name='69/694230/Dimerization_domain/3'> dimerization domain</scene>. This domain connects and stabilizes the two pseudosymmetric dimers and is composed of the <scene name='69/694230/Alpha_1/1'>α1 helix</scene>, the C-terminus of the <scene name='69/694230/Alpha_five/1'>α5 helix</scene> , and the <scene name='69/694230/Alpha_6/1'>α6 helix</scene>. This domain is connected to the DNA binding domain by the long α5 helix. The DNA binding domain stabilizes the major and minor groove of DNA via the <scene name='69/694230/Whth_4/5'>winged helix-turn-helix (wHTH)</scene> motif. Binding of Zinc allows AdcR to bind DNA and activate the transcription of high-affinity Zinc specific uptake transporters. The binding of Zinc to the <scene name='69/694230/2_binding_sites/2'>Zinc binding pocket</scene> induces a conformational change that allows for a hydrogen bond network between helices of the binding domain. It is believed that this hydrogen bond network is the allosteric activator needed to expose residues that bind the bases along the major groove of the DNA <ref name="guerra">PMID:22085181</ref>. The binding sites are found adjacent to the DNA binding domain.

Sandbox Reserved 1063: Difference between revisions

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Revision as of 18:13, 21 April 2017 view source Alexi Zaniker (talk \| contribs) 94 edits No edit summary ← Older edit		Revision as of 18:13, 21 April 2017 view source Isaac C. Gluesenkamp (talk \| contribs) 69 edits No edit summary Newer edit →
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	==='''Structural Overview'''===		==='''Structural Overview'''===
	One of the two functional domains of AdcR is <scene name='69/694230/Dimerization_domain/3'> dimerization domain</scene>. This domain connects and stabilizes the two pseudosymmetric dimers and is composed of the <scene name='69/694230/Alpha_1/1'>α1 helix</scene>, the C-terminus of the <scene name='69/694230/Alpha_five/1'>α5 helix</scene> , and the <scene name='69/694230/Alpha_6/1'>α6 helix</scene>. This domain is connected to the DNA binding domain by the long α5 helix. The DNA binding domain stabilizes the major and minor groove of DNA via the winged helix-turn-helix motif. Binding of Zinc allows AdcR to bind DNA and activate the transcription of high-affinity Zinc specific uptake transporters. The binding of Zinc to the <scene name='69/694230/2_binding_sites/2'>Zinc binding pocket</scene> induces a conformational change that allows for a hydrogen bond network between helices of the binding domain. It is believed that this hydrogen bond network is the allosteric activator needed to expose residues that bind the bases along the major groove of the DNA <ref name="guerra">PMID:22085181</ref>. The binding sites are found adjacent to the DNA binding domain.		One of the two functional domains of AdcR is <scene name='69/694230/Dimerization_domain/3'> dimerization domain</scene>. This domain connects and stabilizes the two pseudosymmetric dimers and is composed of the <scene name='69/694230/Alpha_1/1'>α1 helix</scene>, the C-terminus of the <scene name='69/694230/Alpha_five/1'>α5 helix</scene> , and the <scene name='69/694230/Alpha_6/1'>α6 helix</scene>. This domain is connected to the DNA binding domain by the long α5 helix. The DNA binding domain stabilizes the major and minor groove of DNA via the <scene name='69/694230/Whth_4/5'>winged helix-turn-helix (wHTH)</scene> motif. Binding of Zinc allows AdcR to bind DNA and activate the transcription of high-affinity Zinc specific uptake transporters. The binding of Zinc to the <scene name='69/694230/2_binding_sites/2'>Zinc binding pocket</scene> induces a conformational change that allows for a hydrogen bond network between helices of the binding domain. It is believed that this hydrogen bond network is the allosteric activator needed to expose residues that bind the bases along the major groove of the DNA <ref name="guerra">PMID:22085181</ref>. The binding sites are found adjacent to the DNA binding domain.