5ec2

KcsA with V76ester+G77dA mutations

spinqualitylabelsall models 5ec2, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ec2 is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DGA, F09, K NonStd Res: DAL, LHV Related: 5ebl, 5ebm, 5ebw, 5ec1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+). Publication Abstract from PubMed The selectivity filter of K(+) channels contains four ion binding sites (S1-S4) and serves dual functions of discriminating K(+) from Na(+) and acting as a gate during C-type inactivation. C-type inactivation is modulated by ion binding to the selectivity filter sites, but the underlying mechanism is not known. Here we evaluate how the ion binding sites in the selectivity filter of the KcsA channel participate in C-type inactivation and in recovery from inactivation. We use unnatural amide-to-ester substitutions in the protein backbone to manipulate the S1-S3 sites and a side-chain substitution to perturb the S4 site. We develop an improved semisynthetic approach for generating these amide-to-ester substitutions in the selectivity filter. Our combined electrophysiological and X-ray crystallographic analysis of the selectivity filter mutants show that the ion binding sites play specific roles during inactivation and provide insights into the structural changes at the selectivity filter during C-type inactivation. Individual Ion Binding Sites in the K(+) Channel Play Distinct Roles in C-type Inactivation and in Recovery from Inactivation.,Matulef K, Annen AW, Nix JC, Valiyaveetil FI Structure. 2016 May 3;24(5):750-61. doi: 10.1016/j.str.2016.02.021. Epub 2016 Apr, 14. PMID:27150040 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 3D structures of antibody

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