5ua3: Difference between revisions

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'''Unreleased structure'''


The entry 5ua3 is ON HOLD  until Paper Publication
==Crystal Structure of a DNA G-Quadruplex with a Cytosine Bulge==
<StructureSection load='5ua3' size='340' side='right' caption='[[5ua3]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ua3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UA3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ua3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ua3 OCA], [http://pdbe.org/5ua3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ua3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ua3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ua3 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polynucleotides containing consecutive tracts of guanines can adopt an intramolecular G-quadruplex structure where multiple planar tetrads of hydrogen-bound guanines stack on top of each other. Remodeling of G-quadruplexes impacts numerous aspects of nucleotide biology including transcriptional and translational control. RNA helicase associated with AU-rich element (RHAU), a member of the ATP-dependent DEX(H/D) family of RNA helicases, has been established as a major cellular quadruplex resolvase. RHAU contains a core helicase domain responsible for ATP binding/hydrolysis/helicase activity and is flanked on either side by N- and C-terminal extensions. The N-terminal extension is required for quadruplex recognition, and we have previously demonstrated complex formation between this domain and a quadruplex from human telomerase RNA. Here we used an integrated approach that includes small angle x-ray scattering, nuclear magnetic resonance spectroscopy, circular dichroism, and dynamic light scattering methods to demonstrate the recognition of G-quadruplexes by the N-terminal domain of RHAU. Based on our results, we conclude that (i) quadruplex from the human telomerase RNA and its DNA analog both adopt a disc shape in solution, (ii) RHAU53-105 adopts a defined and extended conformation in solution, and (iii) the N-terminal domain mediates an interaction with a guanine tetrad face of quadruplexes. Together, these data form the foundation for understanding the recognition of quadruplexes by the N-terminal domain of RHAU.


Authors: Meier, M., McDougall, M.D., Krahn, N.J., Torres, A.M., McRae, E.K.S., Booy, E.P., Patel, T.R., McKenna, S.A., Stetefeld, J.
Binding of G-quadruplexes to the N-terminal recognition domain of the RNA helicase associated with AU-rich element (RHAU).,Meier M, Patel TR, Booy EP, Marushchak O, Okun N, Deo S, Howard R, McEleney K, Harding SE, Stetefeld J, McKenna SA J Biol Chem. 2013 Dec 6;288(49):35014-27. doi: 10.1074/jbc.M113.512970. Epub 2013, Oct 22. PMID:24151078<ref>PMID:24151078</ref>


Description: Crystal Structure of a DNA G-Quadruplex with a Cytosine Bulge
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Mcrae, E.K.S]]
<div class="pdbe-citations 5ua3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Booy, E P]]
[[Category: Krahn, N J]]
[[Category: McDougall, M D]]
[[Category: McKenna, S A]]
[[Category: McRae, E K.S]]
[[Category: Meier, M]]
[[Category: Patel, T R]]
[[Category: Stetefeld, J]]
[[Category: Stetefeld, J]]
[[Category: Mckenna, S.A]]
[[Category: Torres, A M]]
[[Category: Booy, E.P]]
[[Category: Cytosine bulge]]
[[Category: Mcdougall, M.D]]
[[Category: Dna]]
[[Category: Meier, M]]
[[Category: Dna structure]]
[[Category: Torres, A.M]]
[[Category: G-quadruplex]]
[[Category: Patel, T.R]]
[[Category: G4]]
[[Category: Krahn, N.J]]
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