5w17: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5w17 is ~~ON HOLD until Paper~~ Publication

==Crystal structure of Campylobacter jejuni YCEI protein that crystallizes with large solvent channels for nanotechnology applications==

== Structural highlights ==

<table><tr><td colspan='2'>[[5w17]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W17 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w17 OCA], [http://pdbe.org/5w17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w17 RCSB], [http://www.ebi.ac.uk/pdbsum/5w17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w17 ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.

~~Authors:~~ Huber, T.R.~~, Snow, C~~.D.

Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.,Huber TR, McPherson EC, Keating CE, Snow CD Bioconjug Chem. 2017 Dec 20. doi: 10.1021/acs.bioconjchem.7b00668. PMID:29232505<ref>PMID:29232505</ref>

~~Description: Crystal structure~~ of ~~Campylobacter jejuni YCEI protein that crystallizes with large solvent channels for nanotechnology applications~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[Category~~: ~~Unreleased Structures]]~~

</div>

[[Category: Huber, T.R]]

[[Category: Snow, C.D]]

== References ==

__TOC__

</StructureSection>

[[Category: Huber, T R]]

[[Category: Snow, C D]]

[[Category: Nanomaterial nanoporous]]

[[Category: Unknown function]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5w17 is ON HOLD  until Paper Publication
+==Crystal structure of Campylobacter jejuni YCEI protein that crystallizes with large solvent channels for nanotechnology applications==
+<StructureSection load='5w17' size='340' side='right' caption='[[5w17]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5w17]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W17 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w17 OCA], [http://pdbe.org/5w17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w17 RCSB], [http://www.ebi.ac.uk/pdbsum/5w17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w17 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (&gt;13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.
-Authors: Huber, T.R., Snow, C.D.
+Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.,Huber TR, McPherson EC, Keating CE, Snow CD Bioconjug Chem. 2017 Dec 20. doi: 10.1021/acs.bioconjchem.7b00668. PMID:29232505<ref>PMID:29232505</ref>
-Description: Crystal structure of Campylobacter jejuni YCEI protein that crystallizes with large solvent channels for nanotechnology applications
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Huber, T.R]]
+<div class="pdbe-citations 5w17" style="background-color:#fffaf0;"></div>
-[[Category: Snow, C.D]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Huber, T R]]
+[[Category: Snow, C D]]
+[[Category: Nanomaterial nanoporous]]
+[[Category: Unknown function]]