5wcu: Difference between revisions
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==Crystal structure of 167 bp nucleosome bound to the globular domain of linker histone H5== | |||
<StructureSection load='5wcu' size='340' side='right' caption='[[5wcu]], [[Resolution|resolution]] 5.53Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5wcu]] is a 22 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCU FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wcu OCA], [http://pdbe.org/5wcu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wcu RCSB], [http://www.ebi.ac.uk/pdbsum/5wcu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wcu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/H5_CHICK H5_CHICK]] Histone H5 performs the same function as H1, being necessary for the condensation of nucleosome chains into higher order structures, and replaces histone H1 in certain cells. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
It has long been suggested that chromatin may form a fiber with a diameter of ~30 nm that suppresses transcription. Despite nearly four decades of study, the structural nature of the 30-nm chromatin fiber and conclusive evidence of its existence in vivo remain elusive. The key support for the existence of specific 30-nm chromatin fiber structures is based on the determination of the structures of reconstituted nucleosome arrays using X-ray crystallography and single-particle cryo-electron microscopy coupled with glutaraldehyde chemical cross-linking. Here we report the characterization of these nucleosome arrays in solution using analytical ultracentrifugation, NMR, and small-angle X-ray scattering. We found that the physical properties of these nucleosome arrays in solution are not consistent with formation of just a few discrete structures of nucleosome arrays. In addition, we obtained a crystal of the nucleosome in complex with the globular domain of linker histone H5 that shows a new form of nucleosome packing and suggests a plausible alternative compact conformation for nucleosome arrays. Taken together, our results challenge the key evidence for the existence of a limited number of structures of reconstituted nucleosome arrays in solution by revealing that the reconstituted nucleosome arrays are actually best described as an ensemble of various conformations with a zigzagged arrangement of nucleosomes. Our finding has implications for understanding the structure and function of chromatin in vivo. | |||
Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures.,Zhou BR, Jiang J, Ghirlando R, Norouzi D, Sathish Yadav KN, Feng H, Wang R, Zhang P, Zhurkin V, Bai Y J Mol Biol. 2018 Sep 14;430(18 Pt B):3093-3110. doi: 10.1016/j.jmb.2018.06.020., Epub 2018 Jun 27. PMID:29959925<ref>PMID:29959925</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5wcu" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Jiang, J S]] | |||
[[Category: Zhou, B R]] | |||
[[Category: 167 bp nucleosome]] | |||
[[Category: 30 nm chromatin fiber]] | |||
[[Category: Chromatin]] | |||
[[Category: Chromatin binding protein-dna complex]] | |||
[[Category: Chromatin folding]] | |||
[[Category: Chromatin higher order structure]] | |||
[[Category: Chromatosome]] | |||
[[Category: Chromosome]] | |||
[[Category: Dna binding]] | |||
[[Category: Gh5]] | |||
[[Category: Globular domain]] | |||
[[Category: Histone fold]] | |||
[[Category: Histone h5]] | |||
[[Category: Linker dna]] | |||
[[Category: Linker histone h5]] | |||
[[Category: Nucleosome binding protein]] | |||
[[Category: Nucleosome core particle]] | |||
[[Category: Nucleosome packing]] | |||
[[Category: Protein dna complex]] | |||
