5wjc: Difference between revisions
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The | ==Crystal structure of Schizosaccharomyces pombe Mis16 in complex with Eic1== | ||
<StructureSection load='5wjc' size='340' side='right' caption='[[5wjc]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5wjc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJC FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjc OCA], [http://pdbe.org/5wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjc RCSB], [http://www.ebi.ac.uk/pdbsum/5wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/HAT2_SCHPO HAT2_SCHPO]] Regulatory subunit of the histone acetylase B (HAT-B) complex (By similarity). The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing (By similarity). Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:15369671, PubMed:24774534, PubMed:24789708, PubMed:25375240). Maintains the deacetylated state of histones specifically in the central core of the centromeres (PubMed:15369671).[UniProtKB:P39984]<ref>PMID:15369671</ref> <ref>PMID:24774534</ref> <ref>PMID:24789708</ref> <ref>PMID:25375240</ref> [[http://www.uniprot.org/uniprot/MIS19_SCHPO MIS19_SCHPO]] Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708, PubMed:25375240). Links mis16 and mis18 to recruit CENP-A through interacting with non-sense-mediated mRNA decay (NMD) factors and the SWI/SNF complex (PubMed:24774534). Links also mis18 with the CCAN/mis6/ctf19 complex to promote CENP-A assembly (PubMed:24789708).<ref>PMID:24774534</ref> <ref>PMID:24789708</ref> <ref>PMID:25375240</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Mis18 complex, composed of Mis16, Eic1, and Mis18 in fission yeast, selectively deposits the centromere-specific histone H3 variant, CENP-A(Cnp1), at centromeres. How the intact Mis18 holo-complex oligomerizes and how Mis16, a well-known ubiquitous histone H4 chaperone, plays a centromere-specific role in the Mis18 holo-complex, remain unclear. Here, we report the stoichiometry of the intact Mis18 holo-complex as (Mis16)2:(Eic1)2:(Mis18)4 using analytical ultracentrifugation. We further determine the crystal structure of Schizosaccharomyces pombe Mis16 in complex with the C-terminal portion of Eic1 (Eic1-CT). Notably, Mis16 accommodates Eic1-CT through the binding pocket normally occupied by histone H4, indicating that Eic1 and H4 compete for the same binding site, providing a mechanism for Mis16 to switch its binding partner from histone H4 to Eic1. Thus, our analyses not only determine the stoichiometry of the intact Mis18 holo-complex but also uncover the molecular mechanism by which Mis16 plays a centromere-specific role through Eic1 association. | |||
Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A(Cnp1)-Specific Assembly Factor through Eic1 Interaction.,An S, Koldewey P, Chik J, Subramanian L, Cho US Structure. 2018 May 1. pii: S0969-2126(18)30137-0. doi:, 10.1016/j.str.2018.04.012. PMID:29804820<ref>PMID:29804820</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5wjc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: An, S]] | [[Category: An, S]] | ||
[[Category: Chik, J]] | [[Category: Chik, J]] | ||
[[Category: Cho, U S]] | |||
[[Category: Koldewey, P]] | |||
[[Category: Subramanian, L]] | [[Category: Subramanian, L]] | ||
[[Category: | [[Category: Eic1 binding]] | ||
[[Category: | [[Category: Fission yeast chaperone for histone h4]] | ||
[[Category: Protein binding]] | |||
[[Category: Subcomponent of mis18 complex]] | |||
[[Category: Wd-40 repeats domain]] | |||
